Pyronine B
Title: Pyronine B
CAS Registry Number: 2150-48-3
CAS Name: 3,6-Bis(diethylamino)xanthylium chloride
Additional Names: [6-(diethylamino)-3H-xanthen-3-ylidene]diethylammonium chloride; C.I. 45010
Molecular Formula: C21H27ClN2O
Molecular Weight: 358.90
Percent Composition: C 70.28%, H 7.58%, Cl 9.88%, N 7.81%, O 4.46%
Literature References: Prepn of metallic complex: DE 54190 (1889 to Bayer); DE 59003 (1891 to A. Leonhardt); Biehringer, Ber. 27, 3299 (1894); J. Prakt. Chem. 54, 217 (1896); Albert, J. Chem. Soc. 1947, 244. Structure: Chamberlin et al., J. Org. Chem. 27, 2263 (1962). The commercial product is a complex with iron as depicted below.
 
Derivative Type: Ferric chloride complex
Properties: Green metallic needles, mp 176-178°. Absorption max (50% ethanol): 555 nm (E1%1cm 2324).
Melting point: mp 176-178°
Absorption maximum: Absorption max (50% ethanol): 555 nm (E1%1cm 2324)
 
Use: Stain for bacteria, molds, ribonucleic acids.

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