Azure A
Title: Azure A
CAS Registry Number: 531-53-3
CAS Name: 3-Amino-7-(dimethylamino)phenothiazin-5-ium chloride
Additional Names: 7-(dimethylamino)-3-imino-3H-phenothiazine hydrochloride; 3-amino-7-dimethylaminophenazathionium chloride; asym-dimethyl-3,7-diaminophenazathionium chloride; asym-dimethylthionine chloride; methylene azure A; C.I. 52005
Molecular Formula: C14H14ClN3S
Molecular Weight: 291.80
Percent Composition: C 57.63%, H 4.84%, Cl 12.15%, N 14.40%, S 10.99%
Literature References: One of the main constituents of methylene azure. Prepn: Bernthsen, Ann. 230, 169 (1885); Kehrmann, Ber. 39, 1804 (1906); MacNeal, Killian, J. Am. Chem. Soc. 48, 740 (1926). Review: H. J. Conn's Biological Stains, R. D. Lillie, Ed. (Williams & Wilkins, Baltimore, 9th ed., 1977) pp 420-421, 603. See also: Colour Index vol. 4 (3rd ed., 1971) p 4469.
Properties: Green glistening crystals or dark green powder. Soluble in water (blue soln); sparingly sol in alc. Absorption max: 620-634 nm (3 ml of a soln of 50 mg in 250 ml H2O diluted to 200 ml; read in a Beckman spectrophotometer 1 cm cell).
Absorption maximum: Absorption max: 620-634 nm (3 ml of a soln of 50 mg in 250 ml H2O diluted to 200 ml; read in a Beckman spectrophotometer 1 cm cell)
Use: Biological stain.

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