Title: Methylene Azure
Additional Names: Azure I
Literature References: A somewhat variable mixture obtained by oxidation of methylene blue, contg primarily azure A and azure B, q.q.v. Prepn: Bernthsen, Ann. 230, 169 (1885); Kehrmann, Ber. 39, 1804 (1906). Review: H. J. Conn's Biological Stains, R. D. Lillie, Ed. (Williams & Wilkins, Baltimore, 9th ed., 1977) pp 493-502.
Properties: Green glistening crystals. Forms blue soln in water; very sparingly sol in alcohol with a reddish brown fluorescence.
Derivative Type: Mixture with methylene blue
Additional Names: Azure II; azure blue II; methylene azure II
Properties: Deep-green powder. Sol in water with blue color, less sol in alcohol, slightly sol in chloroform. Insol in ether.
Derivative Type: Mixture with methylene blue and eosin
Additional Names: Azure II eosin
Properties: Chief ingredient of Giemsa stain. Green powder. Slightly sol in water; sol in alcohol, methanol, glycerol.
Use: Biological stain.