Sudan Black B
Title: Sudan Black B
CAS Registry Number: 4197-25-5
CAS Name: 2,3-Dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthalenyl]azo]-1H-perimidine
Additional Names: C.I. Solvent Black 3; C.I. 26150; SBB; SSB; SBB-II; SSB-II
Molecular Formula: C29H24N6
Molecular Weight: 456.54
Percent Composition: C 76.29%, H 5.30%, N 18.41%
Literature References: Lipid dye comprised of two major blue components SBB-I, SBB-II and at least 12 secondary components. SBB-II is defined by the chemical name and CAS registry number cited. Introduced: L. Lison, C.R. Seances Soc. Biol. Ses Fil. 115, 202 (1934). TLC purification and histochemistry: A. G. W. Lansink, Histochemie 16, 68 (1968); and solvent effects: W. M. Frederiks et al., Acta Histochem. Suppl. 24, 259 (1981). Structure elucidation: U. Pfüller et al., Histochemistry 54, 237 (1977). Staining technique: D. A. Nelson, F. R. Davey, "Sudan Black B Staining" in Hematology, W. J. Williams et al., Eds. (McGraw-Hill Publishing Co., New York, 1990) pp 1747-1748. Use as lipid stain: H. N. Subramaniam, K. A. Chaubal, J. Biochem. Biophys. Methods 21, 9 (1990).
Properties: Basic compound completely decomposed at 180-186°.
 
Derivative Type: Sudan Black B I
Additional Names: SBB-I; SSB-I; 2,3-dihydro-2,2-dimethyl-4[(4-phenylazo-1-naphthalenyl)-azo]-1H-perimidine
Properties: Neutral compound, mp 180-186° (dec). Sublimates 140-160° at 10-3 torr without decompositon.
Melting point: mp 180-186° (dec)
 
Use: Biological stain for lipids.

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