Tetrazolium Blue
Title: Tetrazolium Blue
CAS Registry Number: 1871-22-3
CAS Name: 3,3¢-(3,3¢-Dimethoxy[1,1¢-biphenyl]-4,4¢-diyl)bis[2,5-diphenyl-2H-tetrazolium] dichloride
Additional Names: 3,3¢-dianisolebis[4,4¢-(3,5-diphenyl)tetrazolium chloride]; blue tetrazolium; dimethoxy neotetrazolium; ditetrazolium chloride; BT
Molecular Formula: C40H32Cl2N8O2
Molecular Weight: 727.64
Percent Composition: C 66.03%, H 4.43%, Cl 9.74%, N 15.40%, O 4.40%
Literature References: Prepn: A. M. Rutenburg et al., Cancer Res. 10, 113 (1950); L. J. Pannone, J. B. Rust, US 2713581 (1955 to Montclair Res. Corp. and Ellis-Foster Co.).
Properties: Lemon-yellow crystals, dec 242-245°. Freely sol in methanol, ethanol, chloroform. Slightly sol in water. Insol in ethyl acetate, acetone, ether. Reduction potential about -0.08 volt. Yields a dark blue diformazan pigment in the presence of a reducing agent.
Use: For research in seed germination, as stain for bacteria and molds, in histochemical studies, to demonstrate oxidation-reduction enzymes in normal and cancerous tissues. See also Triphenyltetrazolium Chloride.

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